Test ID: ANCAR
Test Name: Anti-Neutrophil Cytoplasmic Antibodies Reflex
Ordering Recommendation
ANCA IgG Indirect immunofluorescence (IFA) testing is not recommended as a first-line screening test for ANCA-associated vasculitis. When ANCA is detected, reflex testing for MPO-ANCA and PR3-ANCA will be performed by ELISA to determine antibody specificity.
Note: ANCA may be positive even in the absence of PR3-ANCA and MPO-ANCA, particularly in various other systemic or inflammatory conditions. ANCA results should be interpreted with caution and in the appropriate clinical context to avoid diagnostic uncertainty.
Test Codes
Specimen Requirements
Specimen Type: Serum
Collection Tube: Serum separator tube (SST) or red top
Collection Instructions: Separate serum from cells ASAP or within 2 hours of collection. Then transfer available serum to a standard transport tube.
Volume Required: 1 mL with a minimum of 500 mcL
Storage/Transport Temperature: Refrigerated/Frozen
Stability: Ambient (up to 8 Hours), refrigerated (up to 2 weeks/14days), frozen (up to 30 days) and avoid freeze/thaw cycles with no more than 3 in total
Unacceptable Conditions: Contaminated, heat-inactivated, containing clots fibrin or gross red blood cells, or severely lipemic hemolyzed or icteric samples
Individual Test Components
Test Code
LAB3115
LAB3114
Method
ELISA or EIA
ELISA or EIA
Reference Interval
Normal Human: <1:40
Test Classification
This test has been cleared by the US Food and Drug Administration and is used per manufacturer's instructions and per Exsera's SOPs. Performance characteristics were verified by Exsera in a manner consistent with CAP/CLIA requirements.
Methodology
Indirect Immunofluorescence assay
Patient Reporting
5 Business Days
Days Performed: M-F
Aliases
ANCAR, Anti-neutrophil cytoplasmic antibodies reflex, MPO, PR3