Test ID: ANCA

• EPIC Code: LAB458
• Exsera Order Code: ANCA
• CPT Code: 86255 (IFA), 86256 (Titer)

Specimen Type: Serum

Collection Tube: Serum separator tube (SST) or red top

Collection Instructions: Separate serum from cells ASAP or within 2 hours of collection. Then transfer available serum to a standard transport tube.

Volume Required: 1 mL with a minimum of 500 mcL  

Storage/Transport Temperature: Refrigerated/Frozen

Stability: Ambient (up to 8 Hours), refrigerated (up to 2 weeks/14days), frozen (up to 30 days) and avoid freeze/thaw cycles with no more than 3 in total

Unacceptable Conditions: Contaminated, heat-inactivated, containing clots fibrin or gross red blood cells, or severely lipemic hemolyzed or icteric samples

Not recommended as a first-line screening test for ANCA-associated vasculitis. Positive ANCA IFA results should be followed by testing for MPO-ANCA and PR3-ANCA to determine antibody specificity. Note that ANCA IFA may be positive in the absence of MPO-ANCA and PR3-ANCA in various other systemic or inflammatory conditions.

Note: When an ANCA (Anti-Neutrophil Cytoplasmic Antibody) test is reported as positive, the C-ANCA or P-ANCA titer is typically included to indicate the antibody concentration.

Normal Human: <1:40

This test has been cleared by the US Food and Drug Administration and is used per manufacturer's instructions and per Exsera's SOPs. Performance characteristics were verified by Exsera in a manner consistent with CAP/CLIA requirements.

Indirect Immunofluorescence assay

5 Business Days

Days Performed: M-F

ANCA, Anti-neutrophil cytoplasmic antibodies 

ANCA- C/P Titer only if positive