Test ID: ANCA

Test Name: Anti-Neutrophil Cytoplasmic Antibodies

Test Codes


  • EPIC Code: LAB458
  • Exsera Order Code: ANCA
  • CPT Code: 86255 (IFA), 86256 (Titer)

Specimen Requirements


Specimen Type: Serum

Collection Tube: Serum separator tube (SST) or red top

Collection Instructions: Separate serum from cells ASAP or within 2 hours of collection. Then transfer available serum to a standard transport tube.

Volume Required: 1 mL with a minimum of 500 mcL  

Storage/Transport TemperatureRefrigerated/Frozen

Stability: Ambient (up to 8 Hours), refrigerated (up to 2 weeks/14days), frozen (up to 30 days) and avoid freeze/thaw cycles with no more than 3 in total

Unacceptable Conditions: Contaminated, heat-inactivated, containing clots fibrin or gross red blood cells, or severely lipemic hemolyzed or icteric samples

Ordering Recommendation


Not recommended as a first-line screening test for ANCA-associated vasculitis. Positive ANCA IFA results should be followed by testing for MPO-ANCA and PR3-ANCA to determine antibody specificity. Note that ANCA IFA may be positive in the absence of MPO-ANCA and PR3-ANCA in various other systemic or inflammatory conditions.

 

 

Note: When an ANCA (Anti-Neutrophil Cytoplasmic Antibody) test is reported as positive, the C-ANCA or P-ANCA titer is typically included to indicate the antibody concentration.

Reference Interval


Normal Human: <1:40

 

Test Classification


This test has been cleared by the US Food and Drug Administration and is used per manufacturer's instructions and per Exsera's SOPs. Performance characteristics were verified by Exsera in a manner consistent with CAP/CLIA requirements.

Methodology


Indirect Immunofluorescence assay

Patient Reporting


5 Business Days

Days Performed: M-F

Aliases


ANCA, Anti-neutrophil cytoplasmic antibodies 

Additional Notes


ANCA- C/P Titer only if positive