Test ID: ANCA+

Comprehensive panel for evaluating ANCA-associated vasculitis. First-line testing includes Myeloperoxidase (MPO) and Serine Proteinase 3 (PR3) antibodies. Indirect immunofluorescence (IFA) ANCA IgG by IFA may be used as an adjunct test when clinical suspicion persists despite negative MPO/PR3 results or when low antibody levels are suspected. For evaluation of autoimmune liver disease, use in conjunction with Autoimmune Liver Disease Panel

Note: As part of this panel, ELISA for MPO and PR3 antibodies will be performed, and ANCA IgG by indirect immunofluorescence (IFA) will also be performed concurrently, regardless of ELISA results.

• EPIC Code: N/A
• Exsera Order Code: ANCA+
• CPT Code: 86255 (IFA), 86256 (Titer)

Specimen Type: Serum

Collection Tube: Serum separator tube (SST) or red top

Collection Instructions: Separate serum from cells ASAP or within 2 hours of collection. Then transfer available serum to a standard transport tube.

Volume Required: 1 mL with a minimum of 500 mcL  

Storage/Transport Temperature: Refrigerated/Frozen

Stability: Ambient (up to 8 Hours), refrigerated (up to 2 weeks/14days), frozen (up to 30 days) and avoid freeze/thaw cycles with no more than 3 in total

Unacceptable Conditions: Contaminated, heat-inactivated, containing clots fibrin or gross red blood cells, or severely lipemic hemolyzed or icteric samples

Normal Human: <1:40

This test has been cleared by the US Food and Drug Administration and is used per manufacturer's instructions and per Exsera's SOPs. Performance characteristics were verified by Exsera in a manner consistent with CAP/CLIA requirements.

See above for individual assays

5 Business Days

Days Performed: M-F

ANCA, Anti-neutrophil cytoplasmic antibodies, MPO (Anti-Myeloperoxidase IgG Antibodies), PR3 (Anti-Proteinase 3 IgG Antibodies)