Test ID: ANAR
Test Name: ANA Reflexive
Ordering Recommendation
Recommended as a first-line screening test for the evaluation of patients with suspected systemic autoimmune rheumatic diseases (also known as ANA-associated rheumatic diseases or connective tissue diseases), such as systemic lupus erythematosus (SLE), Sjögren syndrome, systemic sclerosis, and mixed connective tissue disease (MCTD).
Note: If ANA is positive, reflex testing will include ANA titer, fluorescence pattern, dsDNA, and ENA testing
Test Codes
- EPIC Code: LAB3465
- Exsera Order Code: ANAR
- CPT Code: 86038 (IFA), 86039 (Titer), 86255 (Cent)
Specimen Requirements
Specimen Type: Serum
Collection Tube: Serum separator tube (SST) or red top
Collection Instructions: Separate serum from cells ASAP or within 2 hours of collection. Then transfer available serum to a standard transport tube.
Volume Required: 1 mL with a minimum of 500 mcL
Storage/Transport Temperature: Refrigerated/Frozen
Stability: Ambient (up to 8 Hours), refrigerated (up to 2 weeks/14days), frozen (up to 30 days) and avoid freeze/thaw cycles with no more than 3 in total
Unacceptable Conditions: Contaminated, heat-inactivated, containing clots fibrin or gross red blood cells, or severely lipemic hemolyzed or icteric samples
Individual Test Components
Test Code
LAB4570
LAB5254
LAB3464
Method
Indirect Immunofluorescence assay
Fluoroenzyme Immunassay
Indirect Immunofluorescence assay
Reference Interval
Normal Human: <1:80
Test Classification
This test has been cleared by the US Food and Drug Administration and is used per manufacturer's instructions and per Exsera's SOPs. Performance characteristics were verified by Exsera in a manner consistent with CAP/CLIA requirements.
Methodology
Indirect Immunofluorescence assay
Patient Reporting
5 Business Days
Days Performed: M-F
Aliases
ANAR, ANA Reflexive, ANA Reflex